Reagents > BioLINE > Allergy

BioLINE Allergy –Line Immuno Assay (LIA) for
Allergy Screening – “The Allergens Shield”

Test Principle
The BioLINE Allergy is a blotting-enzyme assay for semi-quantitative measurement of allergen-specific IgE antibodies in human sera and used as an aid in the diagnosis of allergy diseases. The principle of the test is shown as below:

1. Specific allergens are bound to the surface of nitrocellulose membranes in lines.
2. Allergen-specific IgE antibodies from the patient’s serum bind to the allergens.
3. Unbound specific IgE and other serum proteins are removed in a washing step.
4. Enzyme-linked anti-IgE antibodies are applied and bind to the allergen-specific IgE antibodies.
5. Unbound enzyme-linked anti-IgE antibodies are removed in a washing step.
6. Substrate is added and catalyzed by alkaline phosphatase into a color signal.

Features and Benefits
1. Menu spanning over hundreds of allergens
(Food, Inhale, Molds, Herbs, etc.)
2. Multiplexing up to 20 allergens per strip
3. Multiple and customizable allergen panels
4. Testing procedure, manual or with automation
5. Cost effective
6. Automation solution with HOB Line

Multiple Panels