Reagents > BioLINE > Autoimmune

BioLINE® Autoimmune –Line Immuno Assay (LIA) for
Auto-antibodies Screening– “Knowing yourself”

Comprehensive panels for the detection of IgG autoantibodies against cell nucleus, vasculitis and Liver antigens. The assays are intended for in vitro diagnostic use only as an aid in the diagnosis of autoimmune diseases such as Systemic Lupus Erythematosus (SLE), Mixed Connective Tissue Disease (MCTD), vasculitis, Primary Biliary Cirrhosis (PBC) and so forth.

Test Principle
Nuclear and associated cytosolic antigens are applied as lines on nitrocellulose membrane strips. The strip is first blocked to prevent nonspecific bindings. During incubation, autoantibodies present in the patient sample bind to the antigens on the strip. An alkaline phosphatase labeled anti-human IgG antibody is used to detect the bound auto-antibodies. After addition of the substrate solution, the appearance of purple blue lines indicates the existence of auto-antibodies against the corresponding antigens.

Features and Benefits
1. Built-in Control line and Cut-off line in each strip for intuitive reagent quality validation and easy result interpretation
2. Stable reagents with excellent compatibility
3. High signal to noise ratio due to minimized background
4. High strip-to-strip and reagent lot-to-lot reproducibility
5. Accurate and objective result interpretation through Software
6. Automation available